DETECTION AND GENOTYPING OF HCV IN PATIENTS OF SHEIKH ZAYED HOSPITAL, LAHORE, PAKISTAN

  • Mohammad Tahir PhD candidate CEMB, University of Punjab
  • Agha Muhammad Suhail Associate professor, HOD Operative Department UM&DC University of Faisalabad
  • Fauzia Tahir Medical Tecnologist, Allama Iqbal Medical College, Lahore
Keywords: HCV, Genotype, RNA, Prevalence, Population, Lahore

Abstract

ABSTRACT
BACKGROUND: A vast majority of chronically infected HCV individuals remain undiagnosed and unaware of their infection status for several years. This study investigated the prevalence of HCV patients in a tertiary-care hospital.
METHODS: Patients attending out-patient department of Sheikh Zayed hospital Lahore were examined during January-March 2012. Diagnosis of Hepatitis C was based on the occurrence of anti-HCV antibodies in serum. HCV RNA infection was confirmed by Real time PCR. Genotypic detection of amplified product was performed on cytoflour. Liver function test (LFT) of ALT was also performed to assess the liver function.
RESULTS: Four hundred and fifty four males and 546 females aged 12-80 years were examined for detection and genotyping of HCV. Sixty nine percent of study population belonged to 30-59 yrs, 26% to ≤29 years, and 5% to ≥60 year old age-groups. Fifty five percent were females. Seventy one percent subjects were found with ALT levels >35. 63% subjects were RNA positive. Forty two percent of males and 58% of females showed genotype 3; In age-groups of ≤19 years, 20-29 years, 30-39 years, 40-49 years, 50-59 years and ≥60 years, genotype 3 was detected in 44%; 51%, 60%, 58%, 58% and 43% respectively. Statistical difference of study variables was nonsignificant (p≥0214) both for age-groups and genders.
CONCLUSION: HCV, genotype 3 highly detected, was equally prevalent in both males and females irrespective of demographic factors. Middle-aged (30-50 years) study population was mostly affected by HCV.

How to Cite
Mohammad Tahir, Agha Muhammad Suhail, & Fauzia Tahir. (1). DETECTION AND GENOTYPING OF HCV IN PATIENTS OF SHEIKH ZAYED HOSPITAL, LAHORE, PAKISTAN. Journal of University Medical & Dental College, 7(1), 25-30. Retrieved from http://jumdc.com/index.php/jumdc/article/view/166
Section
Original Articles