Mutational pattern in Capsid Protein (C) of Dengue Virus isolates from Punjab Province of Pakistan

Mutational pattern in Capsid Protein (C) of Dengue Virus

  • Saira Musshtaq Associate Professor, Department of Biochemistry, Aziz Fatimah Medical & Dental College, Faisalabad.
  • Muhammad Tahir Khan Zhongjing Research and Industrialization Institute of Chinese Medicine, Zhongguancun Scientific Park, Meixi, Nanyang, Henan, China
  • Malik Ihsan Ullah Khan Assistant Professor, Department of Biochemistry, Institute of Molecular Biology and Biotechnology, The University of Lahore.
  • Maira Mahmood Associate Professor, Department of Biochemistry, FMH College of Medicine and Dentistry, Lahore.
  • Farhat Humayun Assistant Professor, Department of Anatomy, Aziz Fatima Medical and Dental college Faisalabad.
  • Aneeqa Shahid Senior Demonstrator, Department of Biochemistry, Aziz Fatima Medical and Dental College, Faisalabad.
Keywords: DENV, Genome, Mutations, Pakistan, Structural proteins, Capsid protein


BACKGROUND & OBJECTIVE: Dengue virus (DENV) genomic study is crucial for understanding how it spreads and presents and become dangerous. Three structural and seven non-structural proteins are encoded by the positive stranded RNA DENV. Capsid protein (C) is one of the structural proteins which helps in the encapsidation of viral RNA. Objective of the study is to identify the DENV serotypes and the most common geographic specific mutations in the C protein, circulating in Punjab province of Pakistan.

METHODOLOGY: It was cross sectional study in which About 120 DENV isolates were selected, by the procedure of temporal sampling done in peak season of dengue fever in 2022, from the major tertiary care hospitals for whole genomes sequence analysis. Among which only 23 whole genomes sequences were performed after virus isolation, quantification and cDNA synthesis.

RESULTS: All the samples were found to be of genotype 1 and 2 in which 44 non-synonymous mutations were detected in the C protein. The most common mutations were; N90S (n=11) and G70S (n=11) followed by V26G (n=5) and R10Q (n=4), in which G70S is novel.

CONCLUSION: Mutations in the current study are of particular interest to design of DENV vaccine projects in future. Genomic epidemiology during each outbreak of DENV in different locations is critical for better public health and for designing new policies for future outbreaks.

How to Cite
Saira Musshtaq, Muhammad Tahir Khan, Malik Ihsan Ullah Khan, Maira Mahmood, Farhat Humayun, & Aneeqa Shahid. (2023). Mutational pattern in Capsid Protein (C) of Dengue Virus isolates from Punjab Province of Pakistan. Journal of University Medical & Dental College, 14(1), 573-579. Retrieved from
Original Article